Tag: biological structures

Lasing achieved with hard X-rays in a resonator

Lasing achieved with hard X-rays in a resonator

Novel “XFELO” laser system produces razor-sharp X-ray light

For the first time, researchers have amplified X-ray light multiple times in a resonator cavity, in a way highly similar to traditional lasers. With great success: the new technique delivers extremely energetic X-ray pulses for high-precision experiments. This development opens up entirely new possibilities for research in physics, chemistry, or biology. The system is called “XFELO”. Researchers from European XFEL, DESY and Hamburg University have published their findings in the latest edition of the journal Nature. 

The team of engineers and scientists have shown for the first time that a hard-X-ray cavity can provide net X-ray gain, with X-ray pulses being circulated between crystal mirrors and amplified in the process, much like happens with an optical laser. The result of the proof-of-concept at European XFEL is a particularly coherent, laser-like light of a quality that is unprecedented in the hard X-ray spectrum. Lasing inside a cavity had been challenging to achieve with short-wavelength X-rays for a variety of reasons, including – on a basic level – that the nature of the light makes it difficult to reflect the beam at large angles. The “XFELO” (short for: X-Ray Free-Electron Laser Oscillator) technique opens new perspectives for scientific investigations, from ultrafast chemical reactions to detailed analyses of the smallest biological structures.

Read more on the European XFEL website

Image: Illustration of the XFELO system: a hard X-ray pulse (red) is reflected by a set of diamond mirrors and oscillates through arrays of magnets, so called undulators. On each roundtrip the pulse meets a new electron bunch (blue), which emits X-rays while passing through the undulators on a slalom course.

Credit: European XFEL

X-raying auditory ossicles – a new technique reveals structures in record time

X-raying auditory ossicles – a new technique reveals structures in record time

Scientists at the Paul Scherrer Institute PSI have refined an X-ray diffraction technique for detecting biological structures from nanometres to millimetres – reducing the time needed to make the measurement from around one day to about an hour. This opens up a wide range of possibilities for biomedical research – from analysing bone and tissue structures to supporting the development of new implants.

Biological materials are masterpieces created by nature. Bones, for example, are extremely hard, yet at the same time elastic enough to withstand lateral forces without breaking easily. This combination of properties results from their hierarchical structure as composite materials – they combine materials that have different structures on different scales. Human-made composite materials are similar in the way they are made up. In reinforced concrete, for example, the concrete component, consisting of cement and sand, can withstand high pressure, while a steel mesh provides high tensile strength and transverse stability. 

Until now, examining such biological materials in detail has required the use of several different instruments, such as electron microscopes or classic light microscopes. However, scientists at the PSI Center for Photon Science have now refined an X-ray diffraction technique that was developed at the institute ten years ago, allowing it to be used to characterise materials on scales from nanometres to millimetres simultaneously and much faster than before. A complete scan now only takes about an hour, instead of a whole day.

To demonstrate the efficiency of their method, the researchers used the Swiss Light Source SLS to reveal the alignment of collagen fibres in a human ossicle known as the incus, or anvil. Collagen fibres are thread-like protein structures that provide tensile strength and elasticity to bones. “In doing so, we have taken the leap from a scientific method to a practical technique,” says Christian Appel, postdoctoral researcher and first author of the study. The results have now been published in the journal Small Methods as its cover story. In future, this method could be valuable in areas such as the study of complex tissue, the analysis of bone diseases and the optimisation of implant designs.

Read more on the PSI website

Image: Scientists at PSI were able to observe the local collagen structures in an ossicle by scanning it with an X-ray beam. The different colours of the cylinders indicate how strongly the collagen bundles are spatially aligned in a section measuring 20 by 20 by 20 micrometres.

Credit: © Paul Scherrer Institute PSI/Christian Appel