New methodology for protein crystallisation

A step-by-step video of the experimental process and associated text protocol to provide a robust method for structure determination by X-ray crystallography of a bacterial protein involved in carbohydrate uptake has been broadcast via the Journal of Visualized Experiments (JOVE).

Scientists from Diamond Light Source have published a novel video-based article of their work here at the synchrotron. The study presented the characterisation of a bacterial protein from Streptococcus pneumoniae that is involved in uptake of carbohydrates by the bacteria to use as a nutrient source. These proteins are potential targets for vaccine and/or new antibacterials due to their location on the surface of the bacteria and their critical role in infection, thus understanding the molecular structure can contribute to new strategies for combatting pneumococcal disease.


Read more on the Diamond website