The new discovery will aid in the development of more efficient and sustainable technologies for bioenergy generation
A study led by researchers from the Brazilian Center for Research in Energy and Materials (CNPEM), located in Campinas (SP), has identified a novel molecular mechanism that explains how enzymes degrade beta-glucans, a class of carbohydrates found in fungi, algae, and plants, with great relevance for industrial and energy applications. The research involved approximately 18 collaborators from the LNBR (Brazilian Biorenewables National Laboratory) and the LNLS (Brazilian Synchrotron Light Laboratory), both part of CNPEM, in addition to external researchers from Unicamp and universities in Spain and Canada.
Published in the scientific journal Nature Communications, the work describes, for the first time, a process called processive catalysis applied to the breakdown of these compounds. In this mechanism, the enzyme acts continuously on the same molecular chain, without detaching itself after each stage of the reaction, which makes the process more efficient.
According to researcher Mariana Morais, one of the study coordinators, the work utilized various techniques and equipment at CNPEM, including directed mutagenesis techniques and kinetic analyses. The research also included high-resolution X-ray crystallography experiments conducted at Sirius, CNPEM’s particle accelerator, as well as computer simulations carried out on the Santos Dumont supercomputer, at the National Laboratory for Scientific Computing (LNCC).
“This integration allowed for the observation, at the atomic level, of all stages of the enzymatic process, from substrate recognition to product release and the restart of the catalytic cycle”, says Morais.
Read more on the CNPEM website
Image: Representation of the enzyme forming a catalytic tunnel that enables interaction with the beta-glucan chain and its continuous processing