The key to why plants flower early in a warming world

Scientists have unveiled a new mechanism that plants use to sense temperature. This finding could lead to solutions to counteract some of the deleterious changes in plant growth, flowering and seed production due to climate change. The results are published today in PNAS.

The rise of temperatures worldwide due to climate change is having detrimental consequences for plants. They tend to flower earlier than before and rush through the reproductive process, which translates into less fruits and less seeds and reduced biomass.

Scientists are now working on the plants’ circadian clock, which determines their growth, metabolism and when they flower. The key thermosensor of the circadian clock is EARLY FLOWERING 3 (ELF3), a protein that plays a vital role in plant development. It integrates various environmental cues, such as light and temperature, with internal developmental signals, to regulate the expression of flowering genes and determine when plants grow and bloom.

A team from the ESRF, CEA and CNRS have determined the molecular mechanism of how ELF3 works in vitro and in the model plant Arabidopsis thaliana. As temperature rises, ELF3 undergoes a process called phase separation. This means that two liquid phases co-exist, in a similar way to oil and water. “We believe that when it goes through phase separation, it sequesters different protein partners like transcription factors, which translates into faster growth and early flowering as a function of elevated temperature”, explains Chloe Zubieta, CNRS Research Director from the Laboratoire de Physiologie Cellulaire et Vegetale at the CEA Grenoble (CNRS/Univ. Grenoble Alpes/CEA/INRAE UMR 5168) and co-corresponding author of the publication. “We are trying to understand the biophysics of the prion-like domain inside ELF3, which we think is the responsible for this phase separation.

ELF3 is a flexible protein, with no well-defined structure, so it cannot be studied using X-ray crystallography, as it needs to be in solution. Instead, the team used mainly Small Angle X-ray Scattering. All existing models showed that the structure would be highly disordered. Then the surprise came up: “I’ve seen many prion-like domains involved in phase separation, but this is the first time I saw something fundamentally different”, explains Mark Tully, ESRF scientist on BM29 and co-corresponding author of the publication.

Read more on the ESRF website