Turning mine waste into healthy soil

Tailings, the waste left after extracting precious and critical minerals, often contain harmful chemicals and heavy metals that can pollute soil, water, and even crops. There are over 1800 tailings storage facilities around the world, and in 2019, a tailings dam in Brazil collapsed; close to 300 people drowned in the waste, which also polluted local land and waterways.

Now a team led by researchers at the University of Queensland has developed an innovative method to turn harmful tailings into healthy soil. The scientists used the Canadian Light Source (CLS) at the University of Saskatchewan to determine the underlying mechanism of their process.

Longbin Huang, a professor with the University of Queensland, said it’s costly and environmentally risky to store tailings over the long term and that other processes for remediating mine waste are slow and extremely expensive. “We have basically taken engineering solutions into the context of natural soil formation from rocks, because tailings have some useful minerals common to natural rocks.” Their solution, he said, could save billions of dollars around the world and carry a host of environmental benefits.

“Tailings have no biologically friendly properties for growing plants. Roots and water cannot penetrate them, and soluble salts and metals in tailings can kill plants and soil microbes,” said Huang. “If you wait for nature to slowly weather the tailings and turn them into soil, it could take a couple thousand years.”

Huang and colleagues found a way to accelerate natural soil formation processes to convert tailings into healthy soil. They recently published their findings in the journal Environmental Science & Technology.

“We can convert these colossal volumes of biologically hostile tailings into growth media similar to natural soil by developing soil structure that will enable biological activity of microbes and plants, basically establishing a natural ecosystem,” he explained.

The process involves encouraging specific microbes to grow in tailings that have been amended with plant mulch from agricultural waste and urban green waste. These microbes “eat” the organics and minerals in tailings, transforming them into functional aggregates (or soil crumbs), the building blocks of healthy soil.  

Read more on CLS website

Battling antibiotic-resistant pathogens one door knob at a time

New antimicrobial coating could revolutionize cleaning methods

We’ve gained a new weapon in the fight against harmful and often antibiotic-resistant pathogens with the development of a unique material engineered to limit disease spread and replace current cumbersome cleaning protocols on high-touch surfaces like door knobs and hand rails.

Using the Canadian Light Source (CLS) at the University of Saskatchewan (USask), researchers from the University of Windsor (UWindsor) have developed and tested a compound of ionic (salt-based) fluids and copper nanoparticles that can coat surfaces and provide germ-free protection that lasts far longer than conventional bleach-based cleaning. For Dr. Abhinandan (Ronnie) Banerjee, the composite material is far superior to “somebody with bleach and a rag trying to keep surfaces sanitized.”

Early in the Covid-19 pandemic, Banerjee and colleagues on the UWindsor’s Trant Team — a research group focused on synthetic bioorganic materials — set their sights on improving sanitizing protocols, which at the time often involved frequent application of compounds like bleach. “The problem with conventional sanitization techniques is it’s not a one-and-done kind of thing,” they said. “It requires a dedicated employee or automation” to keep surfaces germ free. Additionally, frequent wiping of a surface can etch the underlying material, creating even more opportunities for pathogens to gather.

Read more on the CLS website

Image: BioXAS Beamline

Credit: Canadian Light Source (CLS)

Understanding sensitive soils to improve quality of surrounding water

Researchers from the Swedish University of Agricultural Sciences in Uppsala are investigating the impact of phosphorous – both that which exists naturally in soil and that which has been added as manure or fertilizer – on sensitive soils and local aquatic systems.

Phosphorus is an essential nutrient for crops and a component of many fertilizers, including animal manure. While it’s critical for plant growth, too much can damage the quality of water bodies near farms. Phosphorus runoff increases the nutrients within aquatic systems that feed algal blooms, which can lead to a decrease in oxygenated water and a reduction of biological diversity in lakes. Algal blooms can impact human health and wildlife as well as the economies of affected communities reliant on fishing and tourism.

“The transfer of phosphorus from land to aquatic recipients is not equally distributed, meaning some parts of the landscape are more vulnerable,” says Faruk Djodjic, Associate Professor at the Department of Aquatic Sciences and Assessment. “By identifying those vulnerable soil profiles and targeting them with mitigation measures, we can improve water and soil quality.”

With the help of the Canadian Light Source (CLS) at the University of Saskatchewan (USask), Djodjic and his colleagues were able to analyze samples to better understand the composition of sensitive soils.

The beamline data from SXRMB helped the researchers identify important compounds that govern phosphorus absorption or release.

Read more on CLS website

Improved treatment for patients with kidney failure

USask researchers have developed a better membrane for dialysis machines that could lead to safer treatment, improved quality of life for patients with kidney failure.

Over two million people worldwide depend on dialysis or a kidney transplant, according to the National Kidney Foundation. In Canada, the number of individuals facing kidney failure has climbed 35 per cent since 2009 and nearly half (46 per cent) of new kidney disease patients are under age 65, according to The Kidney Foundation of Canada.

Using the Canadian Light Source (CLS) at the University of Saskatchewan (USask), researchers have developed a better membrane for dialysis machines that could lead to safer treatment and improved quality of life for patients with kidney failure.

A dialysis machine is used to filter toxins, waste products, salts, and excess fluid from a patient’s blood when their kidneys can no longer perform this function well. However, negative reactions between dialysis membranes and the patient’s blood can lead to serious complications like blood clots, heart conditions, anemia, blood poisoning, infections, and more.

Dr. Amira Abdelrasoul, an associate professor with USask’s College of Engineering, is an expert on membranes and is determined to help patients on dialysis. “I lost a close family member due to dialysis,” she said. “I saw all the complications he experienced and how he suffered. So, I put all my efforts, knowledge, and background into this research area because I would like to support patients and avoid anyone having to lose a loved one from this treatment.”

The new dialysis membrane developed by her team is a significant improvement over those used in hospitals today, according to Abdelrasoul. Some of the commercial membranes currently in use contain heparin, a medicine that reduces blood clots; however, they also have an intense negative charge on their surface that causes serious side effects.

Read more on the CLS website

Newly identified protein could help fight cancer

Researchers from the University of British Columbia (UBC) have identified a new protein that helps an oral bacterium thrive in other locations around the body. The discovery could eventually lead to the development of new drugs that specifically target the protein.

“This bacterium is common in the mouths of humans and generally doesn’t cause disease in that location. However, it can travel through the bloodstream to other areas of the body, which leads to some pretty big health concerns,” says Dr. Kirsten Wolthers, Associate Professor of Biochemistry and Microbiology at UBC’s Okanagan Campus.

Most notably, this bacteria is prevalent in the tumors of colorectal cancer patients. The presence of the bacteria can contribute to tumor growth, spread of cancer to other sites in the body, and resistance to chemotherapy.

With the help of the CMCF beamline at the Canadian Light Source (CLS), located at the University of Saskatchewan, Wolthers and her colleagues determined that the new protein they identified enables the bacteria to take essential nutrients, such as iron, from our blood cells.

Read more on the CLS website

Image: Alexis Gauvin, inspecting a protein sample for particulate matter, using the glove box. Gauvin is a biochemistry student and a member of Dr. Kirsten Wolthers’s research group in the Department of Chemistry, University of British Columbia (Okanagan Campus).

Building a better carbon capture system

Carbon capture has been hailed as a ground-breaking technology for cleaning the air. And it is, but there are some drawbacks – it’s expensive, and most technology requires the generation and application of heat, which creates emissions.

There had to be a better way, thought Dr. Haotian Wang, associate professor in the Department of Chemical and Biomolecular Engineering at Rice University at Houston, Texas.

Wang and his team found it in a process of electrolysis they studied at Rice and collaborated on with the Canadian Light Source (CLS) at the University of Saskatchewan. They have devised a modular solid electrolyte reactor that, in time, will be usable everywhere, in industry but also for “household use, small business use, space station, submarine, any enclosed environment,” he said. Their study was published in the journal Nature.

“Our new approach is integrated capture and regeneration, which means that you can continuously concentrate the carbon dioxide from dilute sources into almost 100 percent purity.”

The reactor is divided into three chambers. Electrolysis, a process by which electric current is passed through a substance to effect a chemical change, occurs on two sides — one performing oxygen reduction and the other oxygen evolution. The oxygen reduction reaction creates an alkaline environment, which captures carbon and then releases it in the central chamber.

The carbon can either be stored underground or converted to valuable products such as alcohols, “which is also an important direction we are working on,” Wang said.

Crucially, no chemical inputs other than water are required and no side products are generated.

Wang has estimated that the cost of capturing carbon will be $83 per ton, but with improvements, that could drop to $58 or even $33 per ton, a big saving from today’s costs, which range from $125 to $600 USD.

“It’s not only the cost but also the energy source that we can use, which is electricity,” he said. “Ideally, we want to transform this into an electrifying process because in the future we can get a lot (of clean electricity) from solar farms, wind farms and nuclear power plants.”

The CLS played an important role in this work.

Read more on the CLS website

Transforming chicken manure into nutrient-rich fertilizer for crops

An international collaboration between researchers from Brazil and the United States has identified a process for turning poultry waste into a soil additive for agriculture.

“Several countries have large poultry production, especially United States and Brazil, where agriculture is also concentrated,” says Aline Leite, a Post Doctoral researcher from the Federal University of Lavras in Brazil. “So, reusing a global residue generated in large amounts is an interesting way of promoting a circular economy.”

The researchers harvested poultry manure from an experimental site in the United States, which they heated to turn into biochar, a carbon-rich substance that is used as a soil additive to replenish critical nutrients like phosphorus.

“We are focused on understanding mechanisms that are responsible for increasing phosphorus availability in materials like manure,” says Leite.

Poultry manure is full of calcium and requires higher temperature treatments to turn the waste into biochar, however, these higher temperatures can have an effect on the amount of phosphorus available.

In order to ensure that the biochar contained sufficient available phosphorus, the researchers enriched it with another mineral, magnesium, which protected the phosphorus from the heat and enabled it to form more soluble forms of phosphorus.

Using the IDEAS and VLS-PGM beamlines at the Canadian Light Source (CLS) at the University of Saskatchewan (USask), the researchers were able to visualize the connection between phosphorus and magnesium and confirm the success of their technique.

Their findings were recently published in the scientific journal, Chemosphere.

While phosphorus reserves are found across the globe, the nutrient is a finite resource. Finding ways to recycle the mineral is an important issue for scientists.

“There’s no excuse for not using the phosphorus that is already in the food chain, for example, by reusing the waste that is already generated,” says Leite.

Leite says that synchrotron technology is essential for research into agricultural applications.

Read more on the Canadian Light Source website

Recycling phosphorus from wastewater to grow better crops

Scientists are helping close the loop on the sustainability cycle with research into nutrient-enhanced biochar — a charcoal-like material made by heating recycled biomass in the absence of oxygen (a process called pyrolysis). Biomass is any living or once-living material – including plants, trees, and animal waste — that can be used as a source of energy.

Daniel Strawn, Professor of Environmental Soil Chemistry at the University of Idaho, and his colleagues are interested in enhancing biochar – which can be used as an amendment to promote soil health — by adding phosphorus, a crucial nutrient for crops.

The research team, which also included scientists from the University of Saskatchewan and Washington State University, has focused its efforts on recovering phosphorus from wastewater.

“Phosphorus is a limited resource, taken out of the ground, processed to produce fertilizer, and eventually it ends up in wastewater,” says Strawn. “We are developing technology to recover it using biochar in a water treatment process.”

Biochar is an effective sponge ­that can soak up phosphorous and other nutrients, like nitrogen, from waterways. The team is testing this treatment process on municipal and agricultural wastewater systems.

With the help of the Canadian Light Source (CLS) at USask, Strawn and his colleagues confirmed in a recent paper which type of phosphorous had been absorbed by the biochar — a crucial step to understanding and refining their process.

Read more on the CLS website

Taking the stress out of growing corn

Global warming is bringing longer growing seasons, allowing producers to expand the types of crops they cultivate. While this can be a benefit, a longer growing season increases the risk of environmental stressors, like frost and cool overnight temperatures.

Researchers from the University of Saskatchewan (USask) are investigating how the first frost impacts corn varieties, in the hopes of finding new ways to maximize crop yields.

“With global climate change, we are realizing that you can push the corn belt northward, so we’re wondering how we can enable the production of that crop by expanding the season,” said Dr. Karen Tanino, professor of abiotic stress physiology in the College of Agriculture and Bioresources.

The team is interested in the cuticular layer of the plant, a defensive barrier that acts like the plant’s skin. If the cuticular layer is more water repellent, it is full of waxy compounds that allow water to run off the plant.

“If water is not present, then the frost is not able to form, which means the plant can avoid that first fall frost,” said Tanino.

With the help of the Mid-IR beamline at the Canadian Light Source (CLS) located at USask, Tanino and her colleagues found that the cool temperatures preceding the first frost influence the composition and quantity of the plant’s cuticle layer, making it more susceptible to freezing temperatures. Their findings were recently published in Physiologia Plantarum.

Read more on the Canadian Light Source website

Ancient fluid in quartz provides key to finding new uranium deposits

Saskatchewan’s Athabasca Basin is home to some of the world’s largest and richest uranium deposits, but it can still be tricky to find them.

Researchers at the University of Regina are studying how the deposits formed more than 1.5 billion years ago to help figure out the best places to look.

“We’re trying to understand the geological factors that control the formation of these deposits so that we know what features we should be looking for to find more uranium resources,” said Dr. Guoxiang Chi, a geologist at the University of Regina.

Chi, his Ph.D. student, Morteza Rabiei, and colleagues used the Canadian Light Source (CLS) at the University of Saskatchewan to analyze samples of quartz from areas known to contain uranium and nearby barren regions, the quartz having formed at the same time as the Athabascan uranium ore. They sliced the quartz into thin sections and studied the tiny droplets of primordial fluid trapped inside. It was from this fluid, circulating through geological fault lines billions of years ago, that today’s uranium ore formed. “By getting information about this paleo-fluid and seeing how it is distributed we can infer where the original uranium came from and what factors control its deposition,” said Chi. Understanding the conditions under which uranium ore is likely to form can help mining companies know where to look.

The results, however, were more complex than expected, he said. Fluid from ore-bearing areas had high levels of uranium, as expected, but so did the fluid from areas with no uranium ore. On the one hand, that is good news as it means that the uranium-rich fluid is more pervasive than first thought, but it also complicates the search for new deposits.

“We were hoping to see a major difference, but found uranium-rich fluid in both places,” he said. “So, if we want to use it as a guide to locate ore, we’ll have to understand the other factors that control deposition.” Chi said those other factors likely involve reducing agents that allow precipitation of the oxidized uranium in the fluid. “Without a reducing agent, you can’t have ore.”

Read more on CLS website

Undermining the foundations of bacterial resistance

Scientists from the University of Guelph have used the Canadian Light Source (CLS) at the University of Saskatchewan to better understand how several infectious bacteria, including E. coli., build a protective sugar-based barrier that helps cloak their cells.

Published in the Journal of Biological Chemistry, the Guelph research provides the very early steps toward new treatments for E. coli and a whole range of bacteria. Their particular focus is on strains of E. coli that cause urinary tract and bloodstream infections, particularly those that are antibiotic resistant.

The research is looking to understand the enzyme that many infectious bacteria use to build the foundations of their protective capsule. The capsule helps shield the bacterium from attack by the human immune system and exists in many clinically distinct variants.

Making vaccines or drugs that targets the capsule itself directly is impractical as such treatments would target only a few bacteria. Instead, the Guelph team is focused on a key enzyme that builds the capsule foundation. This foundation could serve as a common point of attack, allowing a single treatment for several key pathogens infecting humans and livestock.

“We are interested in the machinery that builds the bacterium’s protective layer,” said Dr. Chris Whitfield, Professor Emeritus in the Department of Molecular and Cellular Biology. “By understanding and targeting the machinery, we can render the pathogen unable to survive in the host”.

Read more on the Canadian Light Source website

Image : Matthew Kimber, Chris Whitfield, and enzyme

New catalyst twice as selective, could make chemical production cleaner and cheaper

An estimated 18 million tonnes of acetic acid are produced annually around the world for industrial applications like making paints, adhesives and coatings. Now, researchers from the University of Toronto (U of T) have demonstrated a new electrically powered catalyst that is twice as efficient as baseline materials at producing acetic acid. Their research has the added bonus of having a much smaller carbon footprint.

Catalysts are used to help convert raw materials into usable products, but the raw materials used to make acetic acid today are fossil fuel-based, meaning production can have negative environmental impacts. Here, the only inputs are CO2-derived CO, water and renewable electricity.

“In this project, I identified a strategy to design catalysts that might be extremely selective to a single chemical, meaning they produce more of the chemical you want, in this case acetic acid, and much less of the by-product chemicals you don’t want,” says Joshua Wicks, a doctoral student in Professor Edward Sargent’s research group at UofT.

“In our lab, we are very interested in the decarbonization of chemicals production and we’re always searching for promising opportunities to apply electrochemistry in this hard-to-decarbonize sector of the economy.”

Read more on the Canadian Light Source website

Image : Panos Papangelakis setting up in-situ XAS experiments

Tiny proteins found across the animal kingdom play a key role in cancer spread

Researchers from McGill University have made an exciting discovery about specific proteins involved in the spread of certain cancers.

Dr. Kalle Gehring, professor of biochemistry and founding director of the McGill Centre for Structural Biology, and his team have focused on unravelling the mystery around phosphatases of regenerating liver (PRLs). These proteins are found in all kinds of animals and insects — from humans to fruit flies – and play a unique role in the growth of cancerous tumours and the spread of cancer throughout the body.

“It’s important for us to study PRLs because they are so important in cancer,” said Gehring, “In some cancers, like metastatic colorectal cancer, the proteins are overexpressed up to 300-fold.”

This overexpression of PRLs makes cancer cells more metastatic and drives the spread to other organs.

In his most recent paper, published in the Journal of Biological Chemistry, Gehring and his colleagues confirmed that PRLs exist in all kinds of single- and multi-cell animals. Data collected at the Canadian Light Source (CLS) at the University of Saskatchewan confirmed the role of PRLs in binding magnesium transporters, helping to further the understanding of how these proteins influence human disease.

Read more on the Canadian Light Source website

Organic matter found in 44-million-year-old beetle fossil

Remember Jurassic Park? The dinosaurs in that movie franchise were brought to life through DNA found in amber. The DNA purportedly came from mosquitoes that had taken blood from dinosaurs prior to being trapped in the tree resin that turned into amber.

Amber, a semi-precious stone that is fossilized tree resin, often contains the fossilized remains of insects and other small creatures, with little, if any, organic matter left. No organic matter, no dinosaur DNA, no Jurassic Park.

However, a team of researchers from the University of Regina, Royal Saskatchewan Museum, and Institute of Life Sciences and Technologies at Daugavpils University in Latvia, have now identified what appears to be organic matter in a 44-million-year-old beetle fossilized in amber.

This remarkable finding, and the methodology used in making it, has been published in Nature’s Scientific Reports, the fifth most-cited journal in the world.

“Using a set of advanced techniques we’ve not tried before, we took a 44-million-year-old beetle trapped in Baltic amber to see if it was possible that any preserved organic material might be present,” says U of R master’s of science student Jerit Mitchell, lead author of the study.

Dr. Mauricio Barbi, a U of R physics professor, says the team used the synchrotron radiation facilities at the University of Saskatchewan’s Canadian Light Source (CLS) in Saskatoon to extract high-resolution 3D micro-computed tomography (micro-CT) images of the beetle.

“The synchrotron mid-infrared radiation gave us the capability to identify possible organic compounds in the specimen. We then complemented these two synchrotron radiation techniques by using a scanning electron microscope to provide further high-resolution images of the beetle and to determine the specific chemical elements present in the sample,” says Barbi, who led the team that discovered structurally preserved fossilized dinosaur cell layers in the skin of a 72-million-year-old hadrosaur.”

Read more on the website

Image: Jerit Mitchell gazing at a millions-year-old fossilized beetle

 Credit: U of R Photography

Researchers study molecular bindings to develop better cancer treatments

A research team based in Winnipeg is using the Canadian Light Source (CLS) at the University of Saskatchewan to find new, cutting-edge ways to battle cancer.

Dr. Jörg Stetefeld, a professor of biochemistry and Tier-1 Canada Research Chair in Structural Biology and Biophysics at the University of Manitoba, is leading groundbreaking research into how netrin-1 — a commonly found molecule related to cell migration and differentiation —  creates filaments and binds to receptors in cells.

As netrin-1 is considered the key player for the migration of cancer cells, Stetefeld said this research could inform new cancer treatments.

“If you understand how netrin binds these receptors, you are sitting in the driver’s seat to develop approaches to block this interaction,” he said. “Why do we want to block it? Because if you block this interaction, you kill the cancer cell.”

Earlier research published in 2016 led to the development of new antibody treatments in Europe for combating breast cancer, said Stetefeld. He hopes this new research, which was published in the journal Nature, can lead to better drugs and treatments as well.

Read more on the CLS website

Imaging Earth’s crust reveals natural secret for reducing carbon emissions

Using the Canadian Light Source (CLS) at the University of Saskatchewan and its BMIT-ID beamline, he discovered much larger pores in samples from the Earth’s crust than predicted.

“I expected nanometer-sized pores, whereas I ended up finding pores up to 200 microns — so several orders of magnitudes larger,” said Pujatti, a scientist in the University of Calgary’s Department of Geoscience who recently defended his PhD. “This was very, very puzzling to me.”

Three-dimensional CLS imaging techniques allowed him to see the rocks’ internal structure. There, he found the pores in a mineral called olivine, which is made up largely of silica and magnesium.

As in other geologic systems, he thought the olivine would form new minerals — basically clays — as it dissolved “but I didn’t see that,” he said. “I could only see pores.”

“Finally, I realized the types of fluids that percolated through these rocks were too cold to lead to the formation of new minerals.” The ‘culprit’ was simply sea water.

“Classically, we always consider the oceanic crust as a sink for magnesium,” he said. “Instead, interactions between fluids and these olivine-rich rocks release magnesium.”

Read more on the Canadian Light Source website

Image: Simone Pujatti (right) and Benjamin Tutolo.